Ginger EST Database

Ginger ( Zingiber officinale) is consumed worldwide as a spice and flavoring agent and is attributed to have many medicinal properties. It is used extensively in Ayurveda, the traditional medicine of India, to block excessive clotting (i.e. heart disease), reduce cholesterol and fight arthritis. Ginger is also extensively consumed as a flavoring agent. It is estimated that in India,the average daily consumption is 8 -10 gm of fresh ginger.

The active ingredients in ginger are thought to reside in its volatile oils, which comprise approximately 1-3% of its weight. The major active ingredients in ginger oil are the sesquiterpenes: bisapolene, zingiberene, and zingiberol. Pharmacological activity of ginger is mainly due to the presence of non-volatile pungent phenolic compounds. The main series of pungent oleoresin compounds present in ginger are known as gingerols of which [6] Gingerols are thought to possess many interesting pharmacological and physiological activities. Despite the pharmacological importance of ginger, its gene expression is still unclear.

The transcriptome of Ginger was analyzed using expressed sequence tag (EST) dataset (38, 169 total) deposited in public domains. Several in silio analysis were done during the annotation process. ESTs were clustered and assembled, resulting in 8624 contigs and 8821 singletons (17,441 unigenes). Assembled dataset was then submitted to the EST annotation pipeline including Blast, Gene Ontology (GO) analysis, and pathway enrichment by Kyoto Encyclopedia of Genes and Genomes (KEGG), Interpro were employed to investigate the functions of the ginger transcriptome. miRNAs (microRNAs) were analyzed and SSRs (Simple Sequence Repeats) were identified using the unigene dataset. Genes involved in gingerol biosynthesis were also identified. The assembly and associated information of transcriptome data provides a comprehensive functional and evolutionary characterization of ginger genomics.